Device
Part:BBa_K142075:Design
Designed by: Julius Rabl Group: iGEM08_ETH_Zurich (2008-10-29)
lac-controlled CFP generator with constitutive lacI expression cassette
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 956
Illegal NheI site found at 979 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2157
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We put the LacI generator behind the lac-controlled expression cassette to minimze readthrough.
Source
Subcloned from BioBricks using standard molecular biology techniques.